rad21 tev Search Results


hap1 rad21 tev cells  (Addgene inc)
96
Addgene inc hap1 rad21 tev cells
Hap1 Rad21 Tev Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hap1 rad21 tev cells/product/Addgene inc
Average 96 stars, based on 1 article reviews
hap1 rad21 tev cells - by Bioz Stars, 2026-02
96/100 stars
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rad21 tev  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology rad21 tev
a) PCR analysis of <t>Rad21</t> alleles from Rad21 Tev/WT , Rad21 Tev/Tev and Rad21 WT/WT mice. b) Schematic of ERt2-TEV-dependent RAD21-TEV degradation c) Western blot of RAD21-TEV protein expression over a time course of 4-OHT treatment. Bar plot of RAD21-TEV protein expression normalised to LAMIN B (n = 7, h = hours). d) Volcano plot of gene expression fold-change versus adjusted P value in RNA-seq of RAD21-TEV neurons transduced with ERt2-TEV and treated with 4-OHT for 24h (n = 3). 463 genes were down- and 287 genes were upregulated (adj P < 0.05 shown in red, DE = differentially expressed). e) Bar graph of individual GO terms and broad categories in RNA-seq of RAD21-TEV neurons treated as in d). Terms represented by downregulated genes are shown in blue. Upregulated genes showed no GO term enrichment at P < 1 × 10 −4 . f) Enrichment of shared deregulated genes (adj P < 0.05) in response to acute cohesin depletion induced by ERt2-TEV and NLS-TEV. Fisher’s exact test was applied for the odds ratio and P value. g) Scatter plot of gene expression, comparing log 2 fold-change of deregulated genes (adj P < 0.05) in response to acute cohesin depletion induced by ERt2-TEV and NLS-TEV (DE = differentially expressed, R = Pearson correlation coefficient). h) 4C analysis of chromatin interactions at the Pcdhb locus. Top: Hi-C representation of domain structure at the Pcdhb locus in mouse cortical neurons , with selected genes and enhancers. Bottom: contact profiles of enhancers HS18-20 in control cells (top panel) and RAD21-TEV cleaved cells (bottom panel). A dashed line indicates the enhancer site and viewpoint. A grey band displays the 20 th to 80 th percentiles and the black line within shows mean values for 40kb windows. The colour panel shows the mean contact intensities for multiple window sizes from 2kb to 5kb, n = 4 independent biological replicates. i) Genes significantly deregulated (adj P < 0.05) by ERt2-TEV mediated RAD21-TEV degradation are enriched for the binding of cohesin (top) CTCF (center) and proximity to neuronal enhancers (bottom, see methods). Fisher’s exact test was applied for the odds ratio and P value. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.
Rad21 Tev, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rad21 tev/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
rad21 tev - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier

Image Search Results


a) PCR analysis of Rad21 alleles from Rad21 Tev/WT , Rad21 Tev/Tev and Rad21 WT/WT mice. b) Schematic of ERt2-TEV-dependent RAD21-TEV degradation c) Western blot of RAD21-TEV protein expression over a time course of 4-OHT treatment. Bar plot of RAD21-TEV protein expression normalised to LAMIN B (n = 7, h = hours). d) Volcano plot of gene expression fold-change versus adjusted P value in RNA-seq of RAD21-TEV neurons transduced with ERt2-TEV and treated with 4-OHT for 24h (n = 3). 463 genes were down- and 287 genes were upregulated (adj P < 0.05 shown in red, DE = differentially expressed). e) Bar graph of individual GO terms and broad categories in RNA-seq of RAD21-TEV neurons treated as in d). Terms represented by downregulated genes are shown in blue. Upregulated genes showed no GO term enrichment at P < 1 × 10 −4 . f) Enrichment of shared deregulated genes (adj P < 0.05) in response to acute cohesin depletion induced by ERt2-TEV and NLS-TEV. Fisher’s exact test was applied for the odds ratio and P value. g) Scatter plot of gene expression, comparing log 2 fold-change of deregulated genes (adj P < 0.05) in response to acute cohesin depletion induced by ERt2-TEV and NLS-TEV (DE = differentially expressed, R = Pearson correlation coefficient). h) 4C analysis of chromatin interactions at the Pcdhb locus. Top: Hi-C representation of domain structure at the Pcdhb locus in mouse cortical neurons , with selected genes and enhancers. Bottom: contact profiles of enhancers HS18-20 in control cells (top panel) and RAD21-TEV cleaved cells (bottom panel). A dashed line indicates the enhancer site and viewpoint. A grey band displays the 20 th to 80 th percentiles and the black line within shows mean values for 40kb windows. The colour panel shows the mean contact intensities for multiple window sizes from 2kb to 5kb, n = 4 independent biological replicates. i) Genes significantly deregulated (adj P < 0.05) by ERt2-TEV mediated RAD21-TEV degradation are enriched for the binding of cohesin (top) CTCF (center) and proximity to neuronal enhancers (bottom, see methods). Fisher’s exact test was applied for the odds ratio and P value. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.

Journal: bioRxiv

Article Title: Partial rescue of neuronal genes deregulated in Cornelia de Lange Syndrome by cohesin

doi: 10.1101/2020.06.06.136432

Figure Lengend Snippet: a) PCR analysis of Rad21 alleles from Rad21 Tev/WT , Rad21 Tev/Tev and Rad21 WT/WT mice. b) Schematic of ERt2-TEV-dependent RAD21-TEV degradation c) Western blot of RAD21-TEV protein expression over a time course of 4-OHT treatment. Bar plot of RAD21-TEV protein expression normalised to LAMIN B (n = 7, h = hours). d) Volcano plot of gene expression fold-change versus adjusted P value in RNA-seq of RAD21-TEV neurons transduced with ERt2-TEV and treated with 4-OHT for 24h (n = 3). 463 genes were down- and 287 genes were upregulated (adj P < 0.05 shown in red, DE = differentially expressed). e) Bar graph of individual GO terms and broad categories in RNA-seq of RAD21-TEV neurons treated as in d). Terms represented by downregulated genes are shown in blue. Upregulated genes showed no GO term enrichment at P < 1 × 10 −4 . f) Enrichment of shared deregulated genes (adj P < 0.05) in response to acute cohesin depletion induced by ERt2-TEV and NLS-TEV. Fisher’s exact test was applied for the odds ratio and P value. g) Scatter plot of gene expression, comparing log 2 fold-change of deregulated genes (adj P < 0.05) in response to acute cohesin depletion induced by ERt2-TEV and NLS-TEV (DE = differentially expressed, R = Pearson correlation coefficient). h) 4C analysis of chromatin interactions at the Pcdhb locus. Top: Hi-C representation of domain structure at the Pcdhb locus in mouse cortical neurons , with selected genes and enhancers. Bottom: contact profiles of enhancers HS18-20 in control cells (top panel) and RAD21-TEV cleaved cells (bottom panel). A dashed line indicates the enhancer site and viewpoint. A grey band displays the 20 th to 80 th percentiles and the black line within shows mean values for 40kb windows. The colour panel shows the mean contact intensities for multiple window sizes from 2kb to 5kb, n = 4 independent biological replicates. i) Genes significantly deregulated (adj P < 0.05) by ERt2-TEV mediated RAD21-TEV degradation are enriched for the binding of cohesin (top) CTCF (center) and proximity to neuronal enhancers (bottom, see methods). Fisher’s exact test was applied for the odds ratio and P value. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.

Article Snippet: Primary antibodies were goat polyclonal to LAMIN B (1:5000, Santa Cruz Biotechnology, sc-6216) and mouse monoclonal to LAMIN B (1:5000, Santa Cruz Biotechnology, sc-374015), and mouse monoclonal anti-myc tag for detection of RAD21-TEV (1:500, Santa Cruz biotechnology, sc-40).

Techniques: Western Blot, Expressing, Gene Expression, RNA Sequencing, Transduction, Hi-C, Control, Binding Assay

a) Immunofluorescence staining for TUJ1 and DAPI in explant RAD21-TEV neurons showing neuronal maturation over the course of ten days (scale bar = 50μm). b) Immunofluorescence staining for NeuN and DAPI in ten-day old explant RAD21-TEV neuron cultures treated with AraC at day 5. Bar graph plots mean % of NeuN+ and NeuN-nuclei in ten-day old explant RAD21-TEV neuron cultures treated with AraC at day 5. (n =10, error bars = range, scale bar = 50μm). c) Immunofluorescence staining for ERt2-TEV and DAPI, with GFP expressed from ERt2-TEV lentiviral transduction. Left: ERT2-TEV is retained in the cytoplasm with vehicle treatment. Right: ERt2-TEV translocates to the nucleus following 4-OHT exposure. White arrow heads indicate example cells. (Scale bar = 25μm).

Journal: bioRxiv

Article Title: Partial rescue of neuronal genes deregulated in Cornelia de Lange Syndrome by cohesin

doi: 10.1101/2020.06.06.136432

Figure Lengend Snippet: a) Immunofluorescence staining for TUJ1 and DAPI in explant RAD21-TEV neurons showing neuronal maturation over the course of ten days (scale bar = 50μm). b) Immunofluorescence staining for NeuN and DAPI in ten-day old explant RAD21-TEV neuron cultures treated with AraC at day 5. Bar graph plots mean % of NeuN+ and NeuN-nuclei in ten-day old explant RAD21-TEV neuron cultures treated with AraC at day 5. (n =10, error bars = range, scale bar = 50μm). c) Immunofluorescence staining for ERt2-TEV and DAPI, with GFP expressed from ERt2-TEV lentiviral transduction. Left: ERT2-TEV is retained in the cytoplasm with vehicle treatment. Right: ERt2-TEV translocates to the nucleus following 4-OHT exposure. White arrow heads indicate example cells. (Scale bar = 25μm).

Article Snippet: Primary antibodies were goat polyclonal to LAMIN B (1:5000, Santa Cruz Biotechnology, sc-6216) and mouse monoclonal to LAMIN B (1:5000, Santa Cruz Biotechnology, sc-374015), and mouse monoclonal anti-myc tag for detection of RAD21-TEV (1:500, Santa Cruz biotechnology, sc-40).

Techniques: Immunofluorescence, Staining, Transduction

a) Schematic of lentiviral construct containing an all-in-one doxycycline inducible NLS-TEV system. Tet-On advanced transactivator (rtTA) and RFP are constitutively expressed under control of the ubiquitin promoter. NLS-TEV expression is in turn controlled by tet response element (TRE) promoter upon addition of doxycycline. b) Schematic of doxycycline dependent RAD21-TEV degradation by NLS-TEV. c) Western blot of RAD21-TEV protein expression 24 hours after Dox pulse (6h, 100ng/ml). Bar plot of RAD21-TEV protein expression normalised to LAMIN B 24 hours after Dox exposure, ~30% RAD21-TEV protein remained (n = 4, h = hours). d) Volcano plot of gene expression log 2 fold-change versus adjusted P value in RAD21-TEV +Dox (24 hours after 6-hour 100ng/ml pulse, n = 3). 570 genes were down- and 150 genes were upregulated (adj P < 0.05, shown in red, DE = differentially expressed). e) Top individual GO terms and broad categories for downregulated genes (blue). There was no significant enrichment for upregulated genes.

Journal: bioRxiv

Article Title: Partial rescue of neuronal genes deregulated in Cornelia de Lange Syndrome by cohesin

doi: 10.1101/2020.06.06.136432

Figure Lengend Snippet: a) Schematic of lentiviral construct containing an all-in-one doxycycline inducible NLS-TEV system. Tet-On advanced transactivator (rtTA) and RFP are constitutively expressed under control of the ubiquitin promoter. NLS-TEV expression is in turn controlled by tet response element (TRE) promoter upon addition of doxycycline. b) Schematic of doxycycline dependent RAD21-TEV degradation by NLS-TEV. c) Western blot of RAD21-TEV protein expression 24 hours after Dox pulse (6h, 100ng/ml). Bar plot of RAD21-TEV protein expression normalised to LAMIN B 24 hours after Dox exposure, ~30% RAD21-TEV protein remained (n = 4, h = hours). d) Volcano plot of gene expression log 2 fold-change versus adjusted P value in RAD21-TEV +Dox (24 hours after 6-hour 100ng/ml pulse, n = 3). 570 genes were down- and 150 genes were upregulated (adj P < 0.05, shown in red, DE = differentially expressed). e) Top individual GO terms and broad categories for downregulated genes (blue). There was no significant enrichment for upregulated genes.

Article Snippet: Primary antibodies were goat polyclonal to LAMIN B (1:5000, Santa Cruz Biotechnology, sc-6216) and mouse monoclonal to LAMIN B (1:5000, Santa Cruz Biotechnology, sc-374015), and mouse monoclonal anti-myc tag for detection of RAD21-TEV (1:500, Santa Cruz biotechnology, sc-40).

Techniques: Construct, Control, Ubiquitin Proteomics, Expressing, Western Blot, Gene Expression

a) Bar graph of overlap between deregulated genes in RAD21-TEV neurons (NLS-TEV and ERt2-TEV combined) and other animal models of neuronal dysfunction including Nipbl +/− (ref. 19), deletion of PRC2 components Ezh1 and Ezh2 ( Ezh1 - 2 −/− ) , Mecp2 +/− (ref. 34), Fmrp −/− (ref. 35), mutant Htt (m Htt ), a mouse model of Huntington’s disease and mouse mutant Pten m3m4/m3m4 (ref. 37). * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001, Fisher’s exact test. b) Bubble plot of number of represented gene ontologies in different mouse models of neuronal dysfunction and RAD21-TEV depleted neurons separated into broad categories. GO analysis conducted on both down- and upregulated genes for each condition indicated in the respective columns. For each condition either all significant GO ( P < 1×10 −4 ) or only top 100, where more than 100 terms reach significance, are plotted. Triangle size is proportional to the number of terms in that category, and direction of point indicates direction of deregulation.

Journal: bioRxiv

Article Title: Partial rescue of neuronal genes deregulated in Cornelia de Lange Syndrome by cohesin

doi: 10.1101/2020.06.06.136432

Figure Lengend Snippet: a) Bar graph of overlap between deregulated genes in RAD21-TEV neurons (NLS-TEV and ERt2-TEV combined) and other animal models of neuronal dysfunction including Nipbl +/− (ref. 19), deletion of PRC2 components Ezh1 and Ezh2 ( Ezh1 - 2 −/− ) , Mecp2 +/− (ref. 34), Fmrp −/− (ref. 35), mutant Htt (m Htt ), a mouse model of Huntington’s disease and mouse mutant Pten m3m4/m3m4 (ref. 37). * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001, Fisher’s exact test. b) Bubble plot of number of represented gene ontologies in different mouse models of neuronal dysfunction and RAD21-TEV depleted neurons separated into broad categories. GO analysis conducted on both down- and upregulated genes for each condition indicated in the respective columns. For each condition either all significant GO ( P < 1×10 −4 ) or only top 100, where more than 100 terms reach significance, are plotted. Triangle size is proportional to the number of terms in that category, and direction of point indicates direction of deregulation.

Article Snippet: Primary antibodies were goat polyclonal to LAMIN B (1:5000, Santa Cruz Biotechnology, sc-6216) and mouse monoclonal to LAMIN B (1:5000, Santa Cruz Biotechnology, sc-374015), and mouse monoclonal anti-myc tag for detection of RAD21-TEV (1:500, Santa Cruz biotechnology, sc-40).

Techniques: Mutagenesis

a) GSEA of RAD21-TEV downregulated genes in CdLS NeuN-positive RNAseq (left). GSEA of CdLS NeuN-positive downregulated genes in RAD21-TEV (right). NES = normalised enrichment score, FDR = false discovery rate. b) Heatmap of log 2 fold-change for selected genes from CdLS NeuN-positive and RAD21-TEV samples separated into broad functional categories. Genes shown were significantly deregulated in CdLS and their mouse orthologs deregulated in at least one RAD21-TEV (ERt2-TEV or NLS-TEV) system. Genes highlighted in red were identified in the SFARI database, those in blue were deregulated in ASD, genes highlighted in orange fulfil both criteria. c) Comparison of RAD21 (cohesin) binding (top), CTCF binding (center) and enhancer proximity (bottom, see methods) of genes deregulated (adj P < 0.05) in NeuN-positive CdLS (left) compared to genes deregulated in both NeuN-positive CdLS and RAD21-TEV (right). Up- and downregulation indicates the direction of deregulation in CdLS NeuN-positive RNAseq. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.

Journal: bioRxiv

Article Title: Partial rescue of neuronal genes deregulated in Cornelia de Lange Syndrome by cohesin

doi: 10.1101/2020.06.06.136432

Figure Lengend Snippet: a) GSEA of RAD21-TEV downregulated genes in CdLS NeuN-positive RNAseq (left). GSEA of CdLS NeuN-positive downregulated genes in RAD21-TEV (right). NES = normalised enrichment score, FDR = false discovery rate. b) Heatmap of log 2 fold-change for selected genes from CdLS NeuN-positive and RAD21-TEV samples separated into broad functional categories. Genes shown were significantly deregulated in CdLS and their mouse orthologs deregulated in at least one RAD21-TEV (ERt2-TEV or NLS-TEV) system. Genes highlighted in red were identified in the SFARI database, those in blue were deregulated in ASD, genes highlighted in orange fulfil both criteria. c) Comparison of RAD21 (cohesin) binding (top), CTCF binding (center) and enhancer proximity (bottom, see methods) of genes deregulated (adj P < 0.05) in NeuN-positive CdLS (left) compared to genes deregulated in both NeuN-positive CdLS and RAD21-TEV (right). Up- and downregulation indicates the direction of deregulation in CdLS NeuN-positive RNAseq. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.

Article Snippet: Primary antibodies were goat polyclonal to LAMIN B (1:5000, Santa Cruz Biotechnology, sc-6216) and mouse monoclonal to LAMIN B (1:5000, Santa Cruz Biotechnology, sc-374015), and mouse monoclonal anti-myc tag for detection of RAD21-TEV (1:500, Santa Cruz biotechnology, sc-40).

Techniques: Functional Assay, Comparison, Binding Assay

Overlap of deregulated genes between RAD21-TEV neurons (NLS-TEV and ERt2-TEV combined) and neuronal or whole cortical tissue RNAseq of human neurological diseases including NeuN-positive CdLS, Rett Syndrome , Huntington’s disease , Alzheimer’s disease , and Down Syndrome . * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001, Fisher’s exact test.

Journal: bioRxiv

Article Title: Partial rescue of neuronal genes deregulated in Cornelia de Lange Syndrome by cohesin

doi: 10.1101/2020.06.06.136432

Figure Lengend Snippet: Overlap of deregulated genes between RAD21-TEV neurons (NLS-TEV and ERt2-TEV combined) and neuronal or whole cortical tissue RNAseq of human neurological diseases including NeuN-positive CdLS, Rett Syndrome , Huntington’s disease , Alzheimer’s disease , and Down Syndrome . * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001, Fisher’s exact test.

Article Snippet: Primary antibodies were goat polyclonal to LAMIN B (1:5000, Santa Cruz Biotechnology, sc-6216) and mouse monoclonal to LAMIN B (1:5000, Santa Cruz Biotechnology, sc-374015), and mouse monoclonal anti-myc tag for detection of RAD21-TEV (1:500, Santa Cruz biotechnology, sc-40).

Techniques:

a) Schematic of NLS-TEV mediated RAD21-TEV degradation and rescue. b) Western blot of RAD21-TEV protein expression over a time course of Dox treatment (6h, 100ng/ml). Bar plot of RAD21-TEV protein expression normalised to LAMIN B (n = 4). c) Volcano plots of gene expression log 2 fold-change versus adjusted P -value in RAD21-TEV +Dox treated neurons before and after rescue of RAD21-TEV expression (n = 3). Left: 570 genes were down- and 150 genes were upregulated (adj P < 0.05, shown in red) following RAD21-TEV depletion. Right: 43 genes were down- and 44 genes were upregulated after rescue of RAD21-TEV expression (adj P < 0.05, shown in red and orange). Genes in red are significantly deregulated in both RAD21-TEV depletion and rescue, genes in orange are significantly deregulated only after rescue (DE = differentially expressed). d) Log 2 fold-change of significantly deregulated genes (adj P < 0.05) following RAD21-TEV depletion (left) and after rescue (right). e) The number of significantly deregulated genes (adj P < 0.05) after RAD21-TEV depletion (in green) and after rescue of RAD21-TEV expression (in orange). DE = differentially expressed. f) Heatmap of de novo deregulated genes following RAD21-TEV rescue (adj P < 0.05) and their maturation trajectory in control (left) and +Dox treated samples (right). g) Barcode plot of de novo deregulated genes following RAD21-TEV rescue (adj P < 0.05) and their enrichment for directionality in maturation. Top: significantly de novo upregulated genes following rescue are enriched for genes upregulated during neuronal maturation. Bottom: de novo downregulated genes after cohesin rescue are enriched for genes that are downregulated during neuronal maturation.

Journal: bioRxiv

Article Title: Partial rescue of neuronal genes deregulated in Cornelia de Lange Syndrome by cohesin

doi: 10.1101/2020.06.06.136432

Figure Lengend Snippet: a) Schematic of NLS-TEV mediated RAD21-TEV degradation and rescue. b) Western blot of RAD21-TEV protein expression over a time course of Dox treatment (6h, 100ng/ml). Bar plot of RAD21-TEV protein expression normalised to LAMIN B (n = 4). c) Volcano plots of gene expression log 2 fold-change versus adjusted P -value in RAD21-TEV +Dox treated neurons before and after rescue of RAD21-TEV expression (n = 3). Left: 570 genes were down- and 150 genes were upregulated (adj P < 0.05, shown in red) following RAD21-TEV depletion. Right: 43 genes were down- and 44 genes were upregulated after rescue of RAD21-TEV expression (adj P < 0.05, shown in red and orange). Genes in red are significantly deregulated in both RAD21-TEV depletion and rescue, genes in orange are significantly deregulated only after rescue (DE = differentially expressed). d) Log 2 fold-change of significantly deregulated genes (adj P < 0.05) following RAD21-TEV depletion (left) and after rescue (right). e) The number of significantly deregulated genes (adj P < 0.05) after RAD21-TEV depletion (in green) and after rescue of RAD21-TEV expression (in orange). DE = differentially expressed. f) Heatmap of de novo deregulated genes following RAD21-TEV rescue (adj P < 0.05) and their maturation trajectory in control (left) and +Dox treated samples (right). g) Barcode plot of de novo deregulated genes following RAD21-TEV rescue (adj P < 0.05) and their enrichment for directionality in maturation. Top: significantly de novo upregulated genes following rescue are enriched for genes upregulated during neuronal maturation. Bottom: de novo downregulated genes after cohesin rescue are enriched for genes that are downregulated during neuronal maturation.

Article Snippet: Primary antibodies were goat polyclonal to LAMIN B (1:5000, Santa Cruz Biotechnology, sc-6216) and mouse monoclonal to LAMIN B (1:5000, Santa Cruz Biotechnology, sc-374015), and mouse monoclonal anti-myc tag for detection of RAD21-TEV (1:500, Santa Cruz biotechnology, sc-40).

Techniques: Western Blot, Expressing, Gene Expression, Control